291 research outputs found
Dose, exposure time, and resolution in Serial X-ray Crystallography
The resolution of X-ray diffraction microscopy is limited by the maximum dose
that can be delivered prior to sample damage. In the proposed Serial
Crystallography method, the damage problem is addressed by distributing the
total dose over many identical hydrated macromolecules running continuously in
a single-file train across a continuous X-ray beam, and resolution is then
limited only by the available molecular and X-ray fluxes and molecular
alignment. Orientation of the diffracting molecules is achieved by laser
alignment. We evaluate the incident X-ray fluence (energy/area) required to
obtain a given resolution from (1) an analytical model, giving the count rate
at the maximum scattering angle for a model protein, (2) explicit simulation of
diffraction patterns for a GroEL-GroES protein complex, and (3) the frequency
cut off of the transfer function following iterative solution of the phase
problem, and reconstruction of an electron density map in the projection
approximation. These calculations include counting shot noise and multiple
starts of the phasing algorithm. The results indicate counting time and the
number of proteins needed within the beam at any instant for a given resolution
and X-ray flux. We confirm an inverse fourth power dependence of exposure time
on resolution, with important implications for all coherent X-ray imaging. We
find that multiple single-file protein beams will be needed for sub-nanometer
resolution on current third generation synchrotrons, but not on fourth
generation designs, where reconstruction of secondary protein structure at a
resolution of 0.7 nm should be possible with short exposures.Comment: 19 pages, 7 figures, 1 tabl
Symbolic Manipulators Affect Mathematical Mindsets
Symbolic calculators like Mathematica are becoming more commonplace among
upper level physics students. The presence of such a powerful calculator can
couple strongly to the type of mathematical reasoning students employ. It does
not merely offer a convenient way to perform the computations students would
have otherwise wanted to do by hand. This paper presents examples from the work
of upper level physics majors where Mathematica plays an active role in
focusing and sustaining their thought around calculation. These students still
engage in powerful mathematical reasoning while they calculate but struggle
because of the narrowed breadth of their thinking. Their reasoning is drawn
into local attractors where they look to calculation schemes to resolve
questions instead of, for example, mapping the mathematics to the physical
system at hand. We model the influence of Mathematica as an integral part of
the constant feedback that occurs in how students frame, and hence focus, their
work
Spin effects in a confined 2DEG: Enhancement of the g-factor, spin-inversion states and their far-infrared absorption
We investigate several spin-related phenomena in a confined two-dimensional
electron gas (2DEG) using the Hartree-Fock approximation for the mutual Coulomb
interaction of the electrons. The exchange term of the interaction causes a
large splitting of the spin levels whenever the chemical potential lies within
a Landau band (LB). This splitting can be reinterpreted as an enhancement of an
effective g-factor, g*. The increase of g* when a LB is half filled can be
accompanied by a spontaneous formation of a static spin-inversion state (SIS)
whose details depend on the system sision state (SIS) whose details depend on
the system size. The coupling of the states of higher LB's into the lowest band
by the Coulomb interaction of the 2DEG is essential for the SIS to occur. The
far-infrared absorption of the system, relatively insensitive to the spin
splitting, develops clear signs of the SIS.Comment: 7 figure
Overview of a Preliminary Destination Mission Concept for a Human Orbital Mission to the Martial Moons
The National Aeronautics and Space Administration s Human Spaceflight Architecture Team (HAT) has been developing a preliminary Destination Mission Concept (DMC) to assess how a human orbital mission to one or both of the Martian moons, Phobos and Deimos, might be conducted as a follow-on to a human mission to a near-Earth asteroid (NEA) and as a possible preliminary step prior to a human landing on Mars. The HAT Mars-Phobos-Deimos (MPD) mission also permits the teleoperation of robotic systems by the crew while in the Mars system. The DMC development activity provides an initial effort to identify the science and exploration objectives and investigate the capabilities and operations concepts required for a human orbital mission to the Mars system. In addition, the MPD Team identified potential synergistic opportunities via prior exploration of other destinations currently under consideration
Narrative exposure therapy for PTSD increases top-down processing of aversive stimuli - evidence from a randomized controlled treatment trial
Adenauer H, Catani C, Gola H, et al. Narrative exposure therapy for PTSD increases top-down processing of aversive stimuli - evidence from a randomized controlled treatment trial. BMC Neuroscience. 2011;12(1): 127.BACKGROUND: Little is known about the neurobiological foundations of psychotherapy for Posttraumatic Stress Disorder (PTSD). Prior studies have shown that PTSD is associated with altered processing of threatening and aversive stimuli. It remains unclear whether this functional abnormality can be changed by psychotherapy. This is the first randomized controlled treatment trial that examines whether narrative exposure therapy (NET) causes changes in affective stimulus processing in patients with chronic PTSD. METHODS: 34 refugees with PTSD were randomly assigned to a NET group or to a waitlist control (WLC) group. At pre-test and at four-months follow-up, the diagnostics included the assessment of clinical variables and measurements of neuromagnetic oscillatory brain activity (steady-state visual evoked fields, ssVEF) resulting from exposure to aversive pictures compared to neutral pictures. RESULTS: PTSD as well as depressive symptom severity scores declined in the NET group, whereas symptoms persisted in the WLC group. Only in the NET group, parietal and occipital activity towards threatening pictures increased significantly after therapy. CONCLUSIONS: Our results indicate that NET causes an increase of activity associated with cortical top-down regulation of attention towards aversive pictures. The increase of attention allocation to potential threat cues might allow treated patients to re-appraise the actual danger of the current situation and, thereby, reducing PTSD symptoms. REGISTRATION OF THE CLINICAL TRIAL: Number: NCT00563888Name: "Change of Neural Network Indicators Through Narrative Treatment of PTSD in Torture Victims" ULR: http://www.clinicaltrials.gov/ct2/show/NCT00563888
Countries with Higher Levels of Gender Equality Show Larger National Sex Differences in Mathematics Anxiety and Relatively Lower Parental Mathematics Valuation for Girls.
Despite international advancements in gender equality across a variety of societal domains, the underrepresentation of girls and women in Science, Technology, Engineering, and Mathematics (STEM) related fields persists. In this study, we explored the possibility that the sex difference in mathematics anxiety contributes to this disparity. More specifically, we tested a number of predictions from the prominent gender stratification model, which is the leading psychological theory of cross-national patterns of sex differences in mathematics anxiety and performance. To this end, we analyzed data from 761,655 15-year old students across 68 nations who participated in the Programme for International Student Assessment (PISA). Most importantly and contra predictions, we showed that economically developed and more gender equal countries have a lower overall level of mathematics anxiety, and yet a larger national sex difference in mathematics anxiety relative to less developed countries. Further, although relatively more mothers work in STEM fields in more developed countries, these parents valued, on average, mathematical competence more in their sons than their daughters. The proportion of mothers working in STEM was unrelated to sex differences in mathematics anxiety or performance. We propose that the gender stratification model fails to account for these national patterns and that an alternative model is needed. In the discussion, we suggest how an interaction between socio-cultural values and sex-specific psychological traits can better explain these patterns. We also discuss implications for policies aiming to increase girls' STEM participation
Phylogenetic and Biogeographic Analysis of Sphaerexochine Trilobites
BACKGROUND: Sphaerexochinae is a speciose and widely distributed group of cheirurid trilobites. Their temporal range extends from the earliest Ordovician through the Silurian, and they survived the end Ordovician mass extinction event (the second largest mass extinction in Earth history). Prior to this study, the individual evolutionary relationships within the group had yet to be determined utilizing rigorous phylogenetic methods. Understanding these evolutionary relationships is important for producing a stable classification of the group, and will be useful in elucidating the effects the end Ordovician mass extinction had on the evolutionary and biogeographic history of the group. METHODOLOGY/PRINCIPAL FINDINGS: Cladistic parsimony analysis of cheirurid trilobites assigned to the subfamily Sphaerexochinae was conducted to evaluate phylogenetic patterns and produce a hypothesis of relationship for the group. This study utilized the program TNT, and the analysis included thirty-one taxa and thirty-nine characters. The results of this analysis were then used in a Lieberman-modified Brooks Parsimony Analysis to analyze biogeographic patterns during the Ordovician-Silurian. CONCLUSIONS/SIGNIFICANCE: The genus Sphaerexochus was found to be monophyletic, consisting of two smaller clades (one composed entirely of Ordovician species and another composed of Silurian and Ordovician species). By contrast, the genus Kawina was found to be paraphyletic. It is a basal grade that also contains taxa formerly assigned to Cydonocephalus. Phylogenetic patterns suggest Sphaerexochinae is a relatively distinctive trilobite clade because it appears to have been largely unaffected by the end Ordovician mass extinction. Finally, the biogeographic analysis yields two major conclusions about Sphaerexochus biogeography: Bohemia and Avalonia were close enough during the Silurian to exchange taxa; and during the Ordovician there was dispersal between Eastern Laurentia and the Yangtze block (South China) and between Eastern Laurentia and Avalonia
Evidence of accelerated ageing in clinical drug addiction from immune, hepatic and metabolic biomarkers
Background: Drug addiction is associated with significant disease and death, but its impact on the ageing process has not been considered. The recent demonstration that many of the items available in routine clinical pathology have applicability as biomarkers of the ageing process implies that routine clinical laboratory parameters would be useful as an initial investigation of this possibility. Methods: 12,093 clinical laboratory results 1995-2006 were reviewed. To make the age ranges of the medical and addicted groups comparable the age range was restricted to 15-45 years. Results: 739 drug addicted (DA) and 5834 general medical (GM) age matched blood samples were compared. Significant elevation of immune parameters was noted in the C-reactive protein, erythrocyte sedimentation rate, total lymphocyte count, serum globulins and the globulin:albumin ratio (P < 0.01). Alanine aminotranferase, creatinine, urea, and insulin like growth factor-1 were also significantly higher (P < 0.01) in the DA group. Albumin, body mass index and dihydroepiandrosterone sulphate were unchanged and cholesterol was lower (all P < 0.05). Conclusion: These data demonstrate for the first time that addiction is associated with an altered profile of common biomarkers of ageing raising the possibility that the ageing process may be altered in this group. Infective and immune processes may be centrally involved. They suggest that addiction forms an interesting model to further examine the contribution of immune suppression and hyperstimulation to the ageing process
- …